Skip to content. | Skip to navigation

Sections
Personal tools
You are here: Home

Search results

33 items matching your search terms.
Filter the results.
Item type


















New items since



Sort by relevance · date (newest first) · alphabetically
File Understanding environmental DNA detection probabilities: A case study using a stream-dwelling char Salvelinus fontinalis
Environmental DNA sampling (eDNA) has emerged as a powerful tool for detecting aquatic animals. Previous research suggests that eDNA methods are substantially more sensitive than traditional sampling. However, the factors influencing eDNA detection and the resulting sampling costs are still not well understood. Here we use multiple experiments to derive independent estimates of eDNA production rates and downstream persistence from brook trout (Salvelinus fontinalis) in streams. We use these estimates to parameterize models comparing the false negative detection rates of eDNA sampling and traditional backpack electrofishing. We find that using the protocols in this study eDNA had reasonable detection probabilities at extremely low animal densities (e.g., probability of detection 0.18 at densities of one fish per stream kilometer) and very high detection probabilities at population-level densities (e.g., probability of detection N0.99 at densities of ≥3 fish per 100 m). This is substantially more sensitive than traditional electrofishing for determining the presence of brook trout and may translate into important cost savings when animals are rare. Our findings are consistent with a growing body of literature showing that eDNA sampling is a powerful tool for the detection of aquatic species, particularly those that are rare and difficult to sample using traditional methods.
Located in Science and Data / Brook Trout Related Publications
File application/x-troff-ms A Protocol for Collecting Environmental DNA Samples From Streams
Environmental DNA (eDNA) is DNA that has been released by an organism into its environment, such that the DNA can be found in air, water, or soil. In aquatic systems, eDNA has been shown to provide a sampling approach that is more sensitive for detecting target organisms faster, and less expensively than previous approaches. However, eDNA needs to be sampled in a manner that has been tested and found effective and, because single copies of target DNA are detected reliably, rigorous procedures must be designed to avoid sample contamination. Here we provide the details of a sampling protocol designed for detecting fish. This protocol, or very similar prototypes, has been used to collect data reported in multiple peer reviewed journal articles and from more than 5,000 additional samples at the time of publication. This process has been shown to be exceedingly sensitive and no case of field contamination has been detected. Over time, we have refined the process to make it more convenient. Our policy at the National Genomics Center for Wildlife and Fish Conservation is to provide collaborators with kits that contain all of the materials necessary to properly collect and store eDNA samples. Although the instructions in this protocol assume that the collaborator will have this same equipment, we also describe how users can create their own kit, and where we think there is flexibility in the equipment used.
Located in Science and Data / Brook Trout Related Publications
File Technical Guide for Field Practitioners: Understanding and Monitoring Aquatic Organism Passage at Road-Stream Crossings
Stream connectivity has become increasingly important for river restoration and fish-habitat improvement projects (Fullerton et al. 2010) amidst increasing evidence that it plays a vital role in supporting aquatic organism populations (Roni et al. 2002; Gibson et al. 2005) and species diversity (Nislow et al. 2011). Recent emphasis on identifying and removing barriers in order to restore aquatic organism passage (AOP) is based on well-documented negative effects of road-stream crossings on fish (Rieman et al. 1997; Hudy et al. 2005) and the potential for cost-effective restoration of aquatic habitat. However, challenges remain in identifying barriers and prioritizing road-stream crossings for remediation. The U.S. Department of Agriculture Forest Service (USFS) has been working to stream-line the process of identifying and remediating road-stream crossings that are inadequate for AOP.
Located in Science and Data / Brook Trout Related Publications
File Notice of Cooperative Agreement Award #F15AC00861
This document describes the conditions for the EBTJV's FY15 Coordination and Operations project supported by FWS-NFHAP funds.
Located in Projects / EBTJV Operational Grants / FY15 EBTJV Coordination and Operations_FWS-NFHAP Funded Agreement
File F15AC00861 Performance Report: 7/30/2015 – 3/31/2017
This report describes accomplishments made under this FWS Service Agreement.
Located in Projects / EBTJV Operational Grants / FY15 EBTJV Coordination and Operations_FWS-NFHAP Funded Agreement
File 2016 MSCGP Grant Application
This document describes the full proposal seeking funding from the 2016 MSCGP.
Located in Projects / EBTJV Operational Grants / 2016 MSCGP Grant
File PS document 2016 MSCGP Grant Scope of Work for Eastern Fish Habitat Partnerships
This document describes the scope of work to be performed by the three Eastern FHPs (ACFHP, SARP, EBTJV) under the 2016 MSCGP grant (Whitewater to Bluewater Initiative).
Located in Projects / EBTJV Operational Grants / 2016 MSCGP Grant
File Troff document 2016 MSCGP Grant Performance Report
This document describes the grant-related accomplishments achieved during the June 1, 2016 – December 31, 2016 performance period.
Located in Projects / EBTJV Operational Grants / 2016 MSCGP Grant
File Joint Meeting of the EBTJV's Conservation Strategy and Science & Data Committees - December 5-7, 2016
This document contains the meeting agenda and a summary of the meeting discussions.
Located in About / EBTJV Partners / EBTJV Partnership Meetings
File Joint Meeting Agenda and Discussion Summary
This documents contains the joint meeting agenda items and summary of discussions.
Located in About / / EBTJV Partnership Meetings / Joint Meeting of EBTJV's Conservation Strategy and Science & Data Committees - February 19, 2016