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File Understanding environmental DNA detection probabilities: A case study using a stream-dwelling char Salvelinus fontinalis
Environmental DNA sampling (eDNA) has emerged as a powerful tool for detecting aquatic animals. Previous research suggests that eDNA methods are substantially more sensitive than traditional sampling. However, the factors influencing eDNA detection and the resulting sampling costs are still not well understood. Here we use multiple experiments to derive independent estimates of eDNA production rates and downstream persistence from brook trout (Salvelinus fontinalis) in streams. We use these estimates to parameterize models comparing the false negative detection rates of eDNA sampling and traditional backpack electrofishing. We find that using the protocols in this study eDNA had reasonable detection probabilities at extremely low animal densities (e.g., probability of detection 0.18 at densities of one fish per stream kilometer) and very high detection probabilities at population-level densities (e.g., probability of detection N0.99 at densities of ≥3 fish per 100 m). This is substantially more sensitive than traditional electrofishing for determining the presence of brook trout and may translate into important cost savings when animals are rare. Our findings are consistent with a growing body of literature showing that eDNA sampling is a powerful tool for the detection of aquatic species, particularly those that are rare and difficult to sample using traditional methods.
Located in Resources / Brook Trout Related Publications
File Technical Guide for Field Practitioners: Understanding and Monitoring Aquatic Organism Passage at Road-Stream Crossings
Stream connectivity has become increasingly important for river restoration and fish-habitat improvement projects (Fullerton et al. 2010) amidst increasing evidence that it plays a vital role in supporting aquatic organism populations (Roni et al. 2002; Gibson et al. 2005) and species diversity (Nislow et al. 2011). Recent emphasis on identifying and removing barriers in order to restore aquatic organism passage (AOP) is based on well-documented negative effects of road-stream crossings on fish (Rieman et al. 1997; Hudy et al. 2005) and the potential for cost-effective restoration of aquatic habitat. However, challenges remain in identifying barriers and prioritizing road-stream crossings for remediation. The U.S. Department of Agriculture Forest Service (USFS) has been working to stream-line the process of identifying and remediating road-stream crossings that are inadequate for AOP.
Located in Resources / Brook Trout Related Publications
File Response of fish assemblages to declining acidic deposition in Adirondack Mountain lakes, 1984-2012
Adverse effects of acidic deposition on the chemistry and fish communities were evident in Adirondack Mountain lakes during the 1980s and 1990s. Fish assemblages and water chemistry in 43 Adirondack Long-Term Monitoring (ALTM) lakes were sampled by the Adirondack Lakes Survey Corporation and the New York State Department of Environmental Conservation during three periods (1984-87, 1994-2005, and 2008-12) to document regional impacts and potential biological recovery associated with the 1990 amendments to the 1963 Clean Air Act (CAA). We assessed standardized data from 43 lakes sampled during the three periods to quantify the response of fish-community richness, total fish abundance, and brook trout (Salvelinus fontinalis) abundance to declining acidity that resulted from changes in U.S. airquality management between 1984 and 2012. During the 28-year period, mean acid neutralizing capacity (ANC) increased significantly from 3 to 30 meq/L and mean inorganic monomeric Al concentrations decreased significantly from 2.22 to 0.66 mmol/L, yet mean species richness, all species or total catch per net night (CPNN), and brook trout CPNN did not change significantly in the 43 lakes. Regression analyses indicate that fishery metrics were not directly related to the degree of chemical recovery and that brook trout CPNN may actually have declined with increasing ANC. While the richness of fish communities increased with increasing ANC as anticipated in several Adirondack lakes, observed improvements in water quality associated with the CAA have generally failed to produce detectable shifts in fish assemblages within a large number of ALTM lakes. Additional time may simply be needed for biological recovery to progress, or else more proactive efforts may be necessary to restore natural fish assemblages in Adirondack lakes in which water chemistry is steadily recovering from acidification.
Located in Resources / Brook Trout Related Publications
File EBTJV Joint Meeting Outcomes and Action Items - 12/16
This document contains a listing of outcomes and action items discussed during the EBTJV's joint Conservation Strategy and Science & Data Committee meeting held in December 2016.
Located in Groups / / Conference Calls / 2017 Steering Committee Conference Call Summaries
File Troff document December 7, 2016 Steering Committee Summary - Draft
This document summarizes the business conducted by the EBTJV Steering Committee during their December 7, 2016 meeting.
Located in Groups / / 2016 Steering Committee Conference Call Summaries / December 7, 2016 EBTJV Steering Committee Meeting Documents
File application/x-maker 2016 June NFH Board Meeting Book
This document contains materials associated with the National Fish Habitat Board's June 29, 2016 meeting that was conducted by teleconference.
Located in Resources / National Fish Habitat Board Meetings / 2016 NFH Board Meetings
File March 2016 NFH Board Meeting
This document contains materials associated with the National Fish Habitat Board's March 7-9, 2016 in-person meeting.
Located in Resources / National Fish Habitat Board Meetings / 2016 NFH Board Meetings
File application/x-maker January 2016 NFH Board Meeting Book
This document contains materials associated with the National Fish Habitat Board's January 20, 2016 meeting that was conducted by teleconference.
Located in Resources / National Fish Habitat Board Meetings / 2016 NFH Board Meetings
File September 20, 2016 Conference Call Summary
This document provides a summary of the EBTJV Steering Committee's discussions that occurred during its teleconference on 9/20/16.
Located in Groups / / 2016 Steering Committee Conference Call Summaries / September 20, 2016 Conference Call Documents
File Joint Meeting Agenda and Discussion Summary
This documents contains the joint meeting agenda items and summary of discussions.
Located in Resources / EBTJV Partnership Meetings / Joint Meeting of EBTJV's Conservation Strategy and Science & Data Committees - February 19, 2016