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File Summary of the 9-20-16 EBTJV Steering Committee Teleconference
This document is a summary of the EBTJV's Steering Committee teleconference that was held on 9-20-16.
Located in Groups / / 2016 Steering Committee Conference Call Summaries / December 7, 2016 EBTJV Steering Committee Meeting Documents
File Technical Guide for Field Practitioners: Understanding and Monitoring Aquatic Organism Passage at Road-Stream Crossings
Stream connectivity has become increasingly important for river restoration and fish-habitat improvement projects (Fullerton et al. 2010) amidst increasing evidence that it plays a vital role in supporting aquatic organism populations (Roni et al. 2002; Gibson et al. 2005) and species diversity (Nislow et al. 2011). Recent emphasis on identifying and removing barriers in order to restore aquatic organism passage (AOP) is based on well-documented negative effects of road-stream crossings on fish (Rieman et al. 1997; Hudy et al. 2005) and the potential for cost-effective restoration of aquatic habitat. However, challenges remain in identifying barriers and prioritizing road-stream crossings for remediation. The U.S. Department of Agriculture Forest Service (USFS) has been working to stream-line the process of identifying and remediating road-stream crossings that are inadequate for AOP.
Located in Science and Data / Brook Trout Related Publications
File Understanding environmental DNA detection probabilities: A case study using a stream-dwelling char Salvelinus fontinalis
Environmental DNA sampling (eDNA) has emerged as a powerful tool for detecting aquatic animals. Previous research suggests that eDNA methods are substantially more sensitive than traditional sampling. However, the factors influencing eDNA detection and the resulting sampling costs are still not well understood. Here we use multiple experiments to derive independent estimates of eDNA production rates and downstream persistence from brook trout (Salvelinus fontinalis) in streams. We use these estimates to parameterize models comparing the false negative detection rates of eDNA sampling and traditional backpack electrofishing. We find that using the protocols in this study eDNA had reasonable detection probabilities at extremely low animal densities (e.g., probability of detection 0.18 at densities of one fish per stream kilometer) and very high detection probabilities at population-level densities (e.g., probability of detection N0.99 at densities of ≥3 fish per 100 m). This is substantially more sensitive than traditional electrofishing for determining the presence of brook trout and may translate into important cost savings when animals are rare. Our findings are consistent with a growing body of literature showing that eDNA sampling is a powerful tool for the detection of aquatic species, particularly those that are rare and difficult to sample using traditional methods.
Located in Science and Data / Brook Trout Related Publications