Perry, Stephen

A Map of Massachusetts Wild Brook Trout Patches: The map provides a visual depiction of the distribution of Massachusetts's wild Brook Trout patches.
Located in Science and Data / … / EBTJV State Maps and Resources / State Maps of Wild Brook Trout Patch Distribution
A Map of New Hampshire Wild Brook Trout Patches: The map provides a visual depiction of the distribution of New Hampshire's wild Brook Trout patches.
Located in Science and Data / … / EBTJV State Maps and Resources / State Maps of Wild Brook Trout Patch Distribution
A Map of North Carolina Wild Brook Trout Patches: The map provides a visual depiction of the distribution of North Carolina's wild Brook Trout patches.
Located in Science and Data / … / EBTJV State Maps and Resources / State Maps of Wild Brook Trout Patch Distribution
A Map of Ohio Wild Brook Trout Patches: The map provides a visual depiction of the distribution of Ohio's wild Brook Trout patches.
Located in Science and Data / … / EBTJV State Maps and Resources / State Maps of Wild Brook Trout Patch Distribution
A Map of South Carolina Brook Trout Patches: The map provides a visual depiction of the distribution of South Carolina's wild Brook Trout patches.
Located in Science and Data / … / EBTJV State Maps and Resources / State Maps of Wild Brook Trout Patch Distribution
A Map of Tennessee Wild Brook Trout Patches: The map provides a visual depiction of the distribution of Tennessee's wild Brook Trout patches.
Located in Science and Data / … / EBTJV State Maps and Resources / State Maps of Wild Brook Trout Patch Distribution
A Map of Virginia Wild Brook Trout Patches: The map provides a visual depiction of the distribution of Virginia's wild Brook Trout patches.
Located in Science and Data / … / EBTJV State Maps and Resources / State Maps of Wild Brook Trout Patch Distribution
A Map of West Virginia Wild Brook Trout Patches: The map provides a visual depiction of the distribution of West Virginia's wild Brook Trout patches.
Located in Science and Data / … / EBTJV State Maps and Resources / State Maps of Wild Brook Trout Patch Distribution
A new species of Myxobolus (Myxozoa: Bivalvulida) infecting the medulla oblongata and nerve cord of brook trout Salvelinus fontinalis in southern Appalachia (New River, NC, USA): Myxobolus neurofontinalis n. sp. infects the brain and medulla oblongata of brook trout (Salvelinus fontinalis [Mitchill, 1814]) in the New River, western NC. It is the first species of Myxobolus described from the brook trout and resembles another congener (Myxobolus arcticus Pugachev and Khokhlov, 1979) that infects nerve tissue of chars (Salvelinus spp.). The new species differs from M. arcticus and all congeners bymyxospore dimensions and by having a mucous envelope and distinctive suturalmarkings. A phylogenetic analysis of the small subunit rDNA (18S) suggests that the new species shares a recent common ancestor with some isolates identified as M. arcticus and that the new species and its close relatives (except Myxobolus insidiosus Wyatt and Pratt, 1973) comprise a clade of salmonid nerve-infecting myxobolids. The phylogenetic analysis indicates that several isolates of “M. arcticus” (sensu lato) in GenBank are misidentified and distantly related to other isolates taken from the type host (Oncorhynchus nerka [Walbaum, 1792]) and from nearby the type locality (Kamchatka Peninsula, Russia). Serial histological sections of infected brook trout confirmed that myxospores of the new species are intercellular and infect nerve cord and medulla oblongata only. A single infected brook trout showed an inflammatory response characterized by focal lymphocytic infiltrates and eosinophilic granulocytes; however, the remaining 4 brook trout lacked evidence of a histopathological change or demonstrable host response. These results do not support the notion that this infection is pathogenic among brook trout.
Located in Science and Data / Brook Trout Related Publications
A Protocol for Collecting Environmental DNA Samples From Streams: Environmental DNA (eDNA) is DNA that has been released by an organism into its environment, such that the DNA can be found in air, water, or soil. In aquatic systems, eDNA has been shown to provide a sampling approach that is more sensitive for detecting target organisms faster, and less expensively than previous approaches. However, eDNA needs to be sampled in a manner that has been tested and found effective and, because single copies of target DNA are detected reliably, rigorous procedures must be designed to avoid sample contamination. Here we provide the details of a sampling protocol designed for detecting fish. This protocol, or very similar prototypes, has been used to collect data reported in multiple peer reviewed journal articles and from more than 5,000 additional samples at the time of publication. This process has been shown to be exceedingly sensitive and no case of field contamination has been detected. Over time, we have refined the process to make it more convenient. Our policy at the National Genomics Center for Wildlife and Fish Conservation is to provide collaborators with kits that contain all of the materials necessary to properly collect and store eDNA samples. Although the instructions in this protocol assume that the collaborator will have this same equipment, we also describe how users can create their own kit, and where we think there is flexibility in the equipment used.
Located in Science and Data / Brook Trout Related Publications

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